Objective?To investigate the clinical effects of percutaneous transhepatic gallbladder fistula combined with choledochoscope and nephroscope in minimally invasive cholecystolithotomy. Methods?Clinical data of 119 cases were divided into two groups. The test group included 57 cases treated by percutaneous transhepatic gallbladder fistula in Minimally Invasive Cholecystolithotomy. The control group included 62 cases treated by Laparoscopy combined with choledochoscopy in minimally invasive cholecystolithotomy. The clinical effects of the two groups were compared. Results?It had no statistical difference in operation time, bleeding, surgical complications, length of hospital stay, gallstone clearance rate and recurrence rate between the two groups.Conclusion?Compared with laparoscopy combined with choledochoscopy, percutaneous transhepatic gallbladder fistula combined with choledochoscope and nephroscope for treatment of cholecystolithiasis was a safe, effective method in the same, with less complications, and important clinical application value.