Objective To investigate the safety, efficacy and advantage of laparoscopic radical cystectomy for male muscle invasive bladder cancer according to three lines and five planes with membrane anatomy.Methods The clinical data of 34 male patients with bladder cancer underwent laparoscopic radical cystectomy from January 2018 to April 2020 were retrospectively analyzed. The average age of the patients was (66.6 ± 7.9) years old (range, 54 ~ 83 years). There were 12 patients with multiple tumors and 22 patients with single tumor, including 3 patients in the triangle region, 6 cases in the left wall, 7 cases in the right wall, 4 cases in the bottom, and 2 cases in the top. The tumor diameter was 1.5～7.5 cm, with an average of (3.6 ± 1.6) cm. The preoperative clinical staging: 20 cases of cT₂N₀M₀, 10 cases of cT₃N₀M₀ and 4 cases of cT_4aN₀M₀. All the operations were performed by the same senior physician, according to the operation idea of three lines and five plans (three lines are ureter, vas deferens and medial umbilical ligament; five planes are Ureteral sheath plane, anterior rectum plane, lateral plane of umbilical artery, pelvic lateral wall plane and posterior pubic plane), using membrane dissection technique, radical cystectomy was performed first, then pelvic lymph node dissection was performed, and finally urinary diversion was performed.Results All the 34 patients completed the operation successfully, the operation time of radical cystectomy and pelvic lymph node dissection was 160～240 min, with an average of (185.6 ± 20.3) min. Intraoperative blood loss was 200～500 mL, and there was no case of blood transfusion. The postoperative pathological examination results: 2 cases of pT₁N₀M₀, 18 cases of pT₂N₀M₀, 11 cases of pT₃N₀M₀, 3 cases of pT_4aN₀M₀, 4 cases of low-grade urothelial carcinoma, 30 cases of high-grade urothelial carcinoma and 1 case of sarcomatoid carcinoma. The surgical margins were negative. There was 1 case of adhesive intestinal obstruction after operation, and the intestinal function recovered after non-surgical treatment; there were no serious surgical complications during and after operation; the postoperative hospital stay was 10～15 days, with an average of (12.5 ± 2.6) days; the postoperative pain score was 1～5 points, with an average of (3.1 ± 1.1) points; the follow-up time was 6～28 months, with an average of (12.5 ± 7.8) months, with no local recurrence and distant metastasis.Conclusion Laparoscopic radical cystectomy based on three lines and five planes with membrane anatomy in treatment of male bladder cancer is safe and effective, which can provide the advantages of clear anatomical marks, distinct organizational planes and controllable complications. It is worthy of extending clinical application.