Objective To explore the application value of Mimics software three-dimensional reconstruction of virtual bronchial tree combined with radial ultrasound in diagnosis of peripheral lung nodules.Methods From January 1, 2020 to February 28, 2021, 23 patients with peripheral solitary pulmonary nodule (SPN) underwent radial-endobronchial ultrasound (R-EBUS) examination, randomized divided into Mimics + R-EBUS group and R-EBUS group. In the R-EBUS group, the nodules were located by hand-painted navigation before operation, the Mimics + R-EBUS group used Mimics software to perform three-dimensional reconstruction of lung nodules and bronchial trees and design inspection paths before surgery. In both groups, rapid on-site evaluation (ROSE) was performed to evaluate the quality of biopsy specimens. Finally, the differences in operation time, complication rate, and diagnosis rate between the two groups were compared.Results The positioning time and operation time of the Mimics+R-EBUS group was shorter than that of the R-EBUS group. There was no statistical difference in the incidence of complications and diagnosis rate between the two groups.Conclusion The personalized virtual bronchial tree based on Mimics three-dimensional reconstruction can provide good positioning and navigation for radial ultrasound bronchoscopy, shorten the operation time, and has clinical application value.