Objective To explore the effective of minimally invasive treatment for double kidney with ureteral duplication with complex renal calculus.Methods From May 2013 to December 2020, 21 cases of double kidney with ureteral duplication with complex renal calculi were treated by percutaneous nephrolithotomy (PCNL), the safety and clinical efficacy were analyzed.Results All the operations were successfully completed and no major complications were noted in all the patients. The average operation time was (80.20 ± 10.25) min, the average hospitalization was (7.30 ± 1.90) d and the estimated blood loss was (85.38 ± 10.14) mL. There were no complications such as infection, channel loss and massive bleeding during and after the operation. 19 cases achieved complete removal of lithotripsy in one stage, 2 cases had residual renal stones, which were completely removed after extracorporeal shock wave lithotripsy, and 1 case had renal arteriovenous fistula, high selective renal artery embolization was performed.Conclusion One stage dual channel PCNL is an effective method for the treatment of double kidney with ureteral duplication with complex renal calculi, and precise puncture technique is the key to success.