Objective To compare the clinical efficacy of the thulium laser en-bloc enucleation of bladder tumor (ThuLEBT) and the plasma transurethral en-bloc enucleation of bladder tumor (TeURBT) in treatment of superficial bladder cancer.Methods 225 patients pathologically identified with non-muscle-invasive bladder cancer were enrolled from September 2018 to September 2020, divided into ThuLEBT group (n = 107) and TeURBT group (n = 118), ThuLEBT group underwent ThuLEBT and TeURBT group underwent TeURBT. The operation time, the incidence of intraoperative obturator nerve reflex, duration of postoperative hematuria, indwelling catheter time, the incidence of bladder irritation, postoperative hospitalization time, and bladder muscle layer was observed in the two groups. After 1 year of postoperative follow-up, gemcitabine intravesical chemotherapy and cystoscopy were performed regularly to record tumor recurrence.Results The two groups of patients have completed the operation, the operation time show no differences between the two groups (P > 0.05); The intraoperative blood loss in the ThuLEBT group was less than that in TeURBT group, postoperative bleeding time, indwelling catheterization time, and postoperative hospitalization time in the ThuLEBT group were shorter than those in TeURBT group, and the differences were statistically significant (P < 0.05); The incidence rate of intraoperative obturator reflex, the incidence of bladder irritation and recurrence rate after 1-year follow-up in ThuLEBT group were lower than those in TeURBT group (P < 0.05); The bladder muscle layer of postoperative pathological examination specimens in ThuLEBT group was significantly higher than that in TeURBT group (P < 0.05).Conclusion ThuLEBT is effective in treatment of non-muscle-invasive bladder cancer, with obvious benefits. It is worthy of being widely popularized in clinical application.