Objective To explore the effect of 3D printing auxiliary stent on the operation of laryngeal microsurgery instrument.Methods Auxiliary stent of suspension laryngoscope was design and 3D printed. 30 standardized training residents as experimental participants were randomly divided into conventional group and auxiliary stent group (15 in each group). The pig’s larynx was used as a specimen, and the vocal folds were marked with localized staining. Participants performed operation on the stained areas of the vocal folds under suspension laryngoscope (60 times of grasping in 1 h). The conventional group performed operation unaided; The auxiliary stent group performed operation with the auxiliary stent as a support. Two senior chief physicians scored the participants' stability and accuracy.Results The operational stability scale score of the conventional group and the auxiliary stent group were (5.03 ± 4.33) and (8.99 ± 3.42), respectively; The operation accuracy rates were 58.4%(526 / 900) and 78.9% (710 / 900), respectively. The operation accuracy rate, operation stability of the auxiliary stent group were significantly higher than those in the conventional group, and the differences were significant (P < 0.01).Conclusion The auxiliary stent can significantly improve the stability and accuracy of operation, which has highly application value.